| 出品公司: | Promega |
|---|---|
| 载体名称: | pNL2.3[secNluc/Hygro] |
| 质粒类型: | 荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 4793 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素 |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 无启动子载体，用于通过分泌的NanoLuc®荧光素酶检测启动子和增强子序列的活性。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pNL2.3[secNlucHygro]载体图谱](/uploads/载体图谱/pNL2.3[secNlucHygro]载体图谱.png)
载体简介
载体序列
LOCUS pNL2.3[secNluc Hygro] 4793 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Promoterless vector for measuring the activity of promoter and
enhancer sequences with a secreted NanoLuc(R) luciferase assay.
ACCESSION JQ513380
VERSION .
KEYWORDS pNL2.3[secNluc Hygro]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4793)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Saturday, June 6, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4793
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
CDS 187..699
/codon_start=1
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
LCERILA"
polyA_signal 748..869
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 1063..1420
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1271..1406
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 1451..2488
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
EVGRV"
polyA_signal 2512..2560
/note="synthetic polyadenylation signal"
rep_origin complement(2887..3475)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3675..4535)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4640..4688
/note="synthetic polyadenylation signal"
misc_feature 4702..4793
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tgaactcctt ctccacaagc
121 gccttcggtc cagttgcctt ctccctgggc ctgctcctgg tgttgcctgc tgccttccct
181 gccccagtct tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac
241 ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc
301 gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat
361 gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt
421 aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg
481 gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc
541 gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt
601 atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga
661 gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaag gccgcgactc tagagtcggg
721 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
781 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
841 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
901 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
961 aaatcgataa ggatccgttt gcgtattggg cgctcttccg ctgatctgcg cagcaccatg
1021 gcctgaaata acctctgaaa gaggaacttg gttagctacc ttctgaggcg gaaagaacca
1081 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
1141 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
1201 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
1261 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
1321 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
1381 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcgatt cttctgacac
1441 tagcgccacc atgaagaagc ccgaactcac cgctaccagc gttgaaaaat ttctcatcga
1501 gaagttcgac agtgtgagcg acctgatgca gttgtcggag ggcgaagaga gccgagcctt
1561 cagcttcgat gtcggcggac gcggctatgt actgcgggtg aatagctgcg ctgatggctt
1621 ctacaaagac cgctacgtgt accgccactt cgccagcgct gcactaccca tccccgaagt
1681 gttggacatc ggcgagttca gcgagagcct gacatactgc atcagtagac gcgcccaagg
1741 cgttactctc caagacctcc ccgaaacaga gctgcctgct gtgttacagc ctgtcgccga
1801 agctatggat gctattgccg ccgccgacct cagtcaaacc agcggcttcg gcccattcgg
1861 gccccaaggc atcggccagt acacaacctg gcgggatttc atttgcgcca ttgctgatcc
1921 ccatgtctac cactggcaga ccgtgatgga cgacaccgtg tccgccagcg tagctcaagc
1981 cctggacgaa ctgatgctgt gggccgaaga ctgtcccgag gtgcgccacc tcgtccatgc
2041 cgacttcggc agcaacaacg tcctgaccga caacggccgc atcaccgccg taatcgactg
2101 gtccgaagct atgttcgggg acagtcagta cgaggtggcc aacatcttct tctggcggcc
2161 ctggctggct tgcatggagc agcagactcg ctacttcgag cgccggcatc ccgagctggc
2221 cggcagccct cgtctgcgag cctacatgct gcgcatcggc ctggatcagc tctaccagag
2281 cctcgtggac ggcaacttcg acgatgctgc ctgggctcaa ggccgctgcg atgccatcgt
2341 ccgcagcggg gccggcaccg tcggtcgcac acaaatcgct cgccggagcg cagccgtatg
2401 gaccgacggc tgcgtcgagg tgctggccga cagcggcaac cgccggccca gtacacgacc
2461 gcgcgctaag gaggtaggtc gagtttaaac tctagaaccg gtcatggccg caataaaata
2521 tctttatttt cattacatct gtgtgttggt tttttgtgtg ttcgaactag atgctgtcga
2581 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
2641 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
2701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
2761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
2821 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
2881 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
2941 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
3001 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
3061 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
3121 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
3181 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
3241 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
3301 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
3361 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
3421 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
3481 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
3541 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
3601 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta
3661 aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta
3721 tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc
3781 ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat
3841 ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg
3901 tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg
3961 agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc
4021 ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata
4081 ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc
4141 gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc
4201 gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata
4261 cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt
4321 actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg
4381 ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct
4441 tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag
4501 ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga
4561 agcatttatc agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg
4621 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
4681 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
4741 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct
//
